Eukaryotic DNA replication relies on a highly orchestrated series of events centred on the Cdc45–MCM–GINS (CMG) helicase complex. Origin licensing begins in G1 phase with loading of a double hexamer ...

Translesion DNA synthesis (TLS) is a specialised branch of the DNA damage tolerance pathway that enables eukaryotic cells to replicate past lesions that would otherwise stall the replication machinery ...

Scientists have discovered that a protein once thought to simply help load a factor necessary for the copying of DNA, actually plays a key role in ensuring fast and reliable replication—an insight ...

DNA replication is a complex process with many moving parts. In baker's yeast, the molecular complex Ctf18-RFC keeps parts of the replication machinery from falling off the DNA strand. Human cells use ...

On their own, however, polymerases aren't good at staying on the DNA strand. They require CTF18-RFC in humans and Ctf18-RFC in yeast to thread a ring-shaped clamp onto the DNA leading strand, and ...

In a new study, published in Cell, researchers describe a newfound mechanism for creating proteins in a giant DNA virus, comparable to a mechanism in eukaryotic cells. The finding challenges the dogma ...