Mycoplasma contamination is prevalent in cell culture labs: in one study, researchers identified mycoplasma contamination in 11% of the 10,000 cell lines they tested (Olarerin-George et al. 2015).

Avoiding contamination is a serious and continual challenge across all cell culture processes. In a previous article, we examined ways of minimizing the impact of chemical contamination resulting from ...

An interview with Patrick Swan, COO of Gold Biotechnology, discussing the effects of mycoplasma contamination on cell cultures and the development of rapid detection kits that provide answers in just ...

Cell culture is a commonly used method to study cells when in vivo study is not possible. It involves growing cells outside of the body of an animal, in controlled settings. These settings should be ...

Cell culture is a deliciously inviting hub for bacteria, fungi, and mycoplasmas. However, as our own 'cell-culture sleuth' points out, failing to detect and eliminate contamination can alter all ...

Even When Less Than Catastrophic, Mycoplasma Infestations Are More Than Inconvenient Bioprocessors have a huge stake in detecting mycoplasma wherever the organism exists in their facilities, but ...

After a microbiology-filled academic and research career, Diana Patzelt became the Global Project Manager for Microbial Enumeration Products at Sartorius (Göttingen, Germany), earning her the title of ...

Mycoplasmas are the smallest known free-living microorganisms and are frequent contaminants of mammalian cell cultures. For the safety and purity of vaccines and other biological cell-derived products ...

A large variety of techniques for the detection of mycoplasma contamination of cell cultures have been described (reviewed in Ref. 1). Many of these methods are lengthy, complex and subjective and ...

Mycoplasma are wall-less bacteria which can be as small as 0.2 – 0.3 µm and can achieve very high densities in cell cultures, ( 10 7 – 10 8 organisms/ml), without discernable pH changes or turbidity.

Mycoplasmas, Acholeplasmas, and Ureaplasmas all belong to the Mollicutes class—small prokaryotes that do not have a rigid cell wall. Contamination of cell culture media by these organisms has become a ...