The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

Figure 1: Enzymatic ligation assisted by nuclease (ELAN) reaction scheme and results. Once DNA fragments with unique and compatible restriction sites have been found or made, DNA molecules can be ...

DNA restriction enzymes, with their widespread applications in many diagnostic procedures (e.g., RFLP, PCR, etc), are the very cornerstone of recombinant DNA technology. Unfortunately, their transport ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

Creative Enzymes, an excellent biotech company specializing in enzyme manufacturing and offering related services, is excited to introduce a new line of restriction enzymes designed to enhance the ...

A new approach by chemists could help to significantly improve diagnostic tests based on PCR. The enzymes used are triggered by light pulses. DNA polymerases and other enzymes that modify DNA are ...

Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at specific sites and then joined together in a controlled manner. They also can be shortened, lengthened, ...